Title: Lactase Lab
Length of Time: 4 days
Objective: Students will know that it takes a reaction (possibly enzyme catalyzed) to convert biomass into a more usable form. Students will analyze and present their findings on the lab they complete to the class on the enzyme action that is affected by an environmental variable.
Standards: Biology Standards: B.1.1, B1.2, B.1.3
Biomass Standards: Students will know that it takes a reaction (possibly enzyme catalyzed) to convert biomass into a more usable form.
21st Century Standards: Collaboration, Critical Thinking, Communication
Materials:
General Procedure:
Assessment: Written lab report at the conclusion of all labs that deal with enzymes and the effects on them by environmental variables.
Length of Time: 4 days
Objective: Students will know that it takes a reaction (possibly enzyme catalyzed) to convert biomass into a more usable form. Students will analyze and present their findings on the lab they complete to the class on the enzyme action that is affected by an environmental variable.
Standards: Biology Standards: B.1.1, B1.2, B.1.3
Biomass Standards: Students will know that it takes a reaction (possibly enzyme catalyzed) to convert biomass into a more usable form.
21st Century Standards: Collaboration, Critical Thinking, Communication
Materials:
- Lactaid tablets: these can be bought in any drug store or grocery store.
- Fifteen milliliters of milk: any milk will work.
- Water: used for dissolving the lactaid tablet, dissolving the sucrose and boiling the lactase.
- Sucrose: five grams per group.
- One hundred milliliter graduated cylinder/ten milliliter graduated cylinder: measuring water and enzyme amounts.
- Three four hundred milliliter beakers: used for dissolving the lactaid tablet, dissolving the sucrose and boiling the lactase.
- Five test tubes: these tubes hold the different solution mixtures.
- Test tube rack: hold the test tubes.
- Marking pencil: mark the test tubes so that confusion does not occur.
- Clock
- Hot plate with a Pyrex test tube for denaturing the enzyme.
- Glucose test strips: these strips can be found in any drugstore
- Glucose meter
- Stirring rod: helps to mix up the lactaid tablet in the water.
General Procedure:
- Students will be measuring the amount of glucose in different milk mixtures.
- They will test the activity of the lactase enzyme in the breakdown of lactose in milk using lactaid tables.
Assessment: Written lab report at the conclusion of all labs that deal with enzymes and the effects on them by environmental variables.
lactase_enzyme_lab.pdf | |
File Size: | 85 kb |
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lactase_enzyme_lab.docx | |
File Size: | 84 kb |
File Type: | docx |
lactase_enzyme_lab.doc | |
File Size: | 48 kb |
File Type: | doc |